That question is hard to answer as you need to factor in power and initiative. You would need to test lineups with two equal players, which is hard to find.
Also, check out space time fights (semi-finals and up) to understand my statement. For example: opponent 1 uses a kushina team against opponent 2 who uses kakuzu team and wins. Opponent 2 beats opponent 1 with a minato team. Both opponent can go back and forth switching their lineups to try to beat each other. There's also times where lineups are determined on when the player uses the mystery.
The question is not difficult to answer what so ever, seal tsunade, kushi and the main and other character used are completely irrelevant.
Unless you come up against someone else also using both of those characters you will win, even if its only by attrition and it goes 10 rounds and you win because tsunades revival gives you more health.
The only stat that matters in this game is initiative, but with two characters as unequivocally broken as kushi and tsunade even that takes a back seat. Theres a reason even the mod with over 1 mil bp in my server gets irritated with bonds these days
Also about the post above mine, this game absolutely only has 1 me
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