You have a fair amount of decent ninjas to work with so there are more combinations than I'll show here, but here are some of the best combinations (in my opinion).

#1 Kushina teams

In terms of pos 1, Kushina is one of your best opinions, even in her 3 star form. These teams are focused around keeping her on the field so she can scale up, with Ino being a good secondary damage dealer and also healer. You can run this team with a bunch of mains, like I'll show here:

If you want to * with fire main you can try this. His shields will help Kushi survive the first round and the red lotus secret passive helps boost Kushi and Ino's power and potentially help reset their mysteries. Along with Kushina's natural cool down from getting hit and Ino's cool down from using her chase, you shouldn't have much issue getting of a mystery most rounds. Gakido will clear debuffs, and Ino will do the same thing as well as heal a bit with her standard. So overall I think it's pretty balanced.

You could also try a variant of this team with other ninjas in place of Gakido, like Sakura [Cloak] or Kurenai (if you have all of Kurenai's breakthroughs). Sakura can be hard to take down because of her ability to gain health when hit, and her attack also scales with each hit, like Kushina. So, the longer the battle lasts, the more valuable she becomes, too. Kurenai is more similar to gakido w/ her mystery, but she can reflect debuffs. It's up to you, you can try them all out. I think Gakido or Sakura would be the most viable. If you wanna use Sakura use the Giant Katsuyu Summon; for Kurenai, the Demon Fox.

You c*so try this team with earth main:

Earth also helps to keep Kushina shielded. He's basically acting like a substitute BT Kazekage Gaara here, who is another ninja who pairs well with Kushi but it looks like you don't have him yet. Again, you c*e the other ninjas I mentioned instead of Gakido if you feel it isn't working out. Just another option. You could also arrange the team so that everyone but Kashina is in the back column and she is in the front middle (the circle marked 5) if you want to get the max out of earth main's mystery turn 1.

Overall, I would suggest investing into getting Kushina to 4 stars. This team will be even better with her breakthroughs and it shouldn't be too expensive in terms of coupons for you since you already have what looks like 1/5 of the frags to get her to 4 star saved already. However, this is solid even now, and I'd recommend going with one of these Kushina teams first since they don't require you to have specific breakthroughs on any of the ninjas.

#2 Shisui Teams

Shisui is another good pos 1 ninja out of your current list. Here's something you can do with him:

This is similar to what used to be a common archetypical team in old metas, except they'd usually have ANBU Itachi instead of Sasuke. This team does assume you have Sasuke fully broken through (honestly, his mystery and standard don't matter as much, but you want Y+2 for his first chase, +2 for his second, and Y+2 for the third). It has good combos and lightning main makes Shisui, Sasuke, and himself immune for two rounds. With Iruka you can stop opposing mysteries round 1 and keep your opponent from setting up. Double barrier with Shisui and Sasuke is a bit weird, but its ok. Ideally you want to keep Shisui's barrier up (as it boosts more of your ninjas) but if his gets overriden, you c*e Sasuke's.

You c*so use this team with fire main, but I think the lightning version is slightly better. But if you want to * with fire main, here you go:

Hope this helps! Let me know if you have any questions!

No problem. Also, this week's Spring Matsuri (wind chimes thing) event is a good place for you to grab some free Kushina frags with the points you get. Good luck!